Composite

Part:BBa_K2536012

Designed by: Anne Byford   Group: iGEM18_Gaston_Day_School   (2018-10-09)


J23116 + GlmY

Glmy with the promoter J23116


GlmYpromoters.png

J23116 + GlmY is significantly more resistant to isobutanol than the control (80.0mM vs 27.48mM; p<0.05). J23116 is a relatively low efficiency promoter and this level of overexpression does not seem to affect the cell membrane as higher efficiency promoters did.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None